The existing study is purposeful on the intracellular pigment extraction from actinomycetes isolated from Kovalam Beach regions of Chennai, Tamil Nadu, India. Only one actinobacterial isolate showed pigmented growth out of total 4 isolates. Ethyl acetate as the solvent was used in cell disruption technique for the extraction of intracellular pigments. UV-Visible spectrophotometry, FT-IR spectroscopy, HPLC and GC-MS were used for the partial characterization of the pigment. The extracted pigment was applied for the preparation of lip balm and assessing its textile dyeing property. In addition, the extracted pigment was analysed for antioxidant, antibacterial activity, MTT assay and haemolytic activity. On optimization, dextrose and maltose were the best carbon sources. The finest nitrogen sources were found to be casein and peptone. The optimum temperature range was 35°C-40°C and optimal pH was found to be between 6.0 and 8.0. The obtained results showed potent antioxidant activity and found to be non-toxic to human erythrocytes. © Published under licence by IOP Publishing Ltd.

Hemalatha S

Department of Software and Systems Engineering

School of Information Technology and Engineering

Vellore Campus

Subathradevi C

Department of Biotechnology

School of Bio Sciences and Technology

Srinivasan M

Merlyn Keziah S

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Pigment from Streptomyces bellus MSA1 isolated from marine sediments

IOP Conference Series: Materials Science and Engineering

Problem statement: Although the diversity of marine Actinobacteria have been studied and biotechnologically exploited throughout the world, the studies on marine actinobacteria in Indian peninsula are largely unexplored. Of 9 maritime states in India, only 4 states have been extensively studied for the diversity of actinobacteria. Further, the studies on bioactive actinomycetes from saline soil are very scanty. In the present study, we had taken an initiative to isolate culturable halophilic actinomycetes and to screen the bioactive potential. Approach: The marine sediment sample was collected at a depth of 400 cm at Marakkanam. The strain was isolated using ISP No. 2 medium supplemented with 25% sea water. The polyphasic taxonomy of the strain was evaluated by phenotypic, chemotaxonomic and phylogenetic analysis. The 16S rRNA was sequenced and phylogenetic relationship with the closest related species were studied. The growth conditions and the medium had been optimized under shake-flask conditions by measuring the dry weight of the mycelium. The isolate was subjected to fermentation and the crude extract was screened for cytotoxic, hemolytic and antimicrobial activity. The cytotoxicity was evaluated on HeLa cells by MTT assay, hemolytic assay on mouse erythrocytes and the antimicrobial activity was determined by agar diffusion assay. Results: Based on polyphasic taxonomy the species was identified as Saccharopolyspora salina and belongs to the genus Saccharopolyspora. The 16S rRNA sequence of the isolate showed 100% similarity with Saccharopolyspora salina. Based on the phylogenetic and phenotypic evaluation the isolate was designated as Saccharopolyspora salina VITSDK4. The growth was maximal in the designed production medium with the incubation temperature of 28°C and pH of 7.4. It is a moderately halophilic species requires 9% NaCl concentration for optimal growth. The cytotoxicity on HeLa cells showed the IC50 value of 26.2 μg mL-1 by MTT assay. The hemolytic activity on mouse erythrocytes showed the EC50 value of 266 μg mL-1. The crude extract also exhibited significant antagonistic activity against fungal pathogens Aspergillus niger, Aspergillus fumigatus, Candida albicans and the Gram negative bacteria Escherichia coli and Klebsiella pneumoniae. Only moderate activity was seen against Gram positive bacteria Staphylococcus aureus and Bacillus subtilis. Conclusion/Recommendations: Based on the results of our investigation, the isolated strain was identified as Saccharopolyspora salina VITSDK4, which was moderately halophilic, produces an extracellular bioactive metabolite, which inhibits the proliferation of HeLa cells as well as antagonistic to fungal and bacterial pathogens. Further studies on purification and characterization of the pure compound from the strain were ongoing. The results of this study suggested that the marine actinomycetes from the unexplored Indian coast could provide lead compounds of therapeutic value. © 2009 Science Publications.

American Journal of Infectious Diseases

Cytotoxic and Antimicrobial Potential of Actinomycete Species Saccharopolyspora salina VITSDK4 Isolated from the Bay of Bengal Coast of India

Asia-Pacific Biotech News

Bioboard

Journal of Pharmaceutical Sciences and Pharmacology

Effectiveness of Medicinal Plants Against Virulent Aeromonas Species from Diverse Sources

European Journal of Lipid Science and Technology

Eur. J. Lipid Sci. Technol. 12/2012

Journal	Data powered by TypesetIOP Conference Series: Materials Science and Engineering
Publisher	Data powered by TypesetIOP Publishing
ISSN	1757-8981
Open Access	No