The incidence of bacterial disease has increased tremendously in the last decade, because of the emergence of drug resistance strains within the bacterial pathogens. The present study was to investigate the antibacterial compound 2,5-di-tert-butyl-1,4-benzoquinone (DTBBQ) isolated from marine Streptomyces sp. VITVSK1 as a potent antibacterial agent. The antibacterial potential of DTBBQ was investigated against RNA Polymerase (PDB ID-1I6V) by in silico molecular docking tools. Results of our study showed the high affinity interaction between DTBBQ and RNA polymerase and also confirmed the drug likeliness of DTBBQ using ADMET in silico pharmacology tools. Our findings suggest that DTBBQ could be used as antibacterial drug to defend the emerging antibacterial resistance. Copyright © 2015 Inderscience Enterprises Ltd.

Kannabiran K

Department of Bio-Medical Sciences

School of Bio Sciences and Technology

Vellore Campus

Jannu V.G

Sanjenbam P

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Preclinical evaluation and molecular docking of 2,5-di-tert-butyl-1,4-benzoquinone DTBBQ from Streptomyces sp. VITVSK1 as a potent antibacterial agent

International Journal of Bioinformatics Research and Applications

Extraction of pigments from natural sources is gaining momentum due to many biological applications. The aim of our study was to extract and to identify the pigment produced by Streptomyces species isolated from saltpan soil samples. The pigment-producing isolate was characterized by molecular taxonomy, identified as Streptomyces species, and designated as Streptomyces sp. VITVSK1. The isolate produced green color pigmentation upon solid substrate fermentation using parboiled rice as a media for 7 days at 37°C. The pigment derivative was extracted using methanol as solvent and purified by silica gel column chromatography and preparative thin layer chromatography using chloroform: methanol as solvent system. The purified compound was identified as 2,5-di-tert-butyl-1,4-benzoquinone (DTBBQ) based on similarity index with reference compounds available in the mass spectra library, NIST. Structure of the pure compound was also elucidated by 1H and 13C nuclear magnetic resonance spectra. The compound DTBBQ showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with IC50 value of 0.6 μg/mL. DTBBQ also showed antimicrobial activity with a zone of inhibition of 21 mm against Bacillus cereus. The results of the present study showed that Streptomyces sp. VITVSK1 could be a promising source for the production of biologically active quinone-based pigments. © 2013 The Korean Society for Applied Biological Chemistry.

Journal of the Korean Society for Applied Biological Chemistry

Extraction of quinone derivative from Streptomyces sp. VITVSK1 isolated from Cheyyur saltpan, Tamilnadu, India

Journal of Antimicrobial Chemotherapy

The 2012 Garrod Lecture: Discovery of antibacterial drugs in the 21st century

Current Opinion in Microbiology

New target for inhibition of bacterial RNA polymerase: ‘switch region’

BMC Bioinformatics

Properties and identification of antibiotic drug targets

Clinical Microbiology Reviews

Bacillus cereus, a Volatile Human Pathogen

Preclinical evaluation and molecular docking of 2,5-di-tert-butyl-1,4-benzoquinone (DTBBQ) from Streptomyces sp. VITVSK1 as a potent antibacterial agent

Journal	International Journal of Bioinformatics Research and Applications
Publisher	Inderscience Publishers
ISSN	1744-5485
Open Access	0