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Preparation and characterization of a Cu (II)-IDA poly HEMA monolith syringe for proteomic applications
Khaparde a, M a v,
Published in Wiley
PMID: 28762518
Volume: 38
Issue: 22-23
Pages: 2981 - 2984

Herein, we report the preparation of a metal-chelate immobilized hydrophilic poly hydroxyethylmethacrylate-based monolith of 100 μL volume in a plastic syringe. The monolith is elastic in nature, contains well interconnected pores with a permeability (k) of 1.3 × 10−12 m2. Immobilization of iminodiacetic acid (IDA) is performed via schiff base reaction. Adsortion of IgG on this copper-IDA monolith is of langmuir isotherm with a maximum adsorption capacity of ∼25 mg IgG per g monolith. IgG adsorption capacity of this affinity monolith remained unaffected with increase of flow rate. This proposed metal-chelate monolith in syringe format has the potential for application in proteomics. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

About the journal
JournalData powered by TypesetELECTROPHORESIS
PublisherData powered by TypesetWiley
Open AccessNo