Dextran is easily soluble, biodegradable and biocompatible biopolymers comprised by glucose units. Due to these characteristics, high purity of dextran serve as important starting and intermediate reagents in a broad range of synthesis in biotechnological and food industries. In this study, Leuconostoc mesenteroides MTCC10508 were obtained from MTCC and were transferred in MRS broth medium at 30°C and pH 6.8 for 24 h. The produced dextran from Leuconostoc mesenteroides MTCC10508 was compared with standard dextran. Furthermore, FT-IR analysis results showed that there were similarities between produced dextran and standard dextran. Produced dextran was cross linked with epichlorohydrin to prepare biopolymer. FT-IR result showed that there was similar bands present between cross linked produced dextran and standard dextran. © RJPT All right reserved.