The aim of the present study was to investigate the hepatoprotective effects of Triphala in D-Galactosamine (D-GalN) induced hepatic toxicity in mice. The mice received a single dose of galactosamine (700mg/kg, i.p) to induce hepatotoxicity; Triphala extract (1000mg/kg, i.p) and silymarin (25 mg/kg, i.p.) were administered after the injection of galactosamine. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), Tumour necrosis factoralpha (TNF α) bilirubin, lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidise (GPx), glutathione reductase (GR), glutathione-s-transferase (GST) and total reduced glutathione were estimated in serum of the mice.It was found that D-GalN induced hepatic damage resulted in a significant (p<0.05) increase in the activity of ALT, AST, ALP, bilirubin, LPO and TNF- αlevel with a decrease in the levels of anti-oxidant enzymes such as SOD, CAT, GPx, GR, GST and Total reduced glutathione which attained normal levels after the treatment of Triphala extract (1000mg/kg/b.wt, i.p). These biochemical observations were supported by histopathological examination of mice liver sections. These observations demonstrate that Triphala treatment may attenuate protective activity against D-galactosamine-induced hepatotoxicity in mice. © 2013 IJDDR, Evan Prince Sabina et al.