Babu S

Department of Biotechnology

School of Bio Sciences and Technology

Vellore Campus

Fulltext

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Biotechnology Journal

We attempted to study the compatibility among plant beneficial bacteria in the culture level by growing them near in the nutrient agar plates. Among all the bacteria tested, Rhizobium was found to inhibit the growth of other bacteria. From the compatible group of PGPR, we have selected one biofertilizer (Azospirillum brasilense strain TNAU) and one biocontrol agent (Pseudomonas fluorescens strain PF1) for further studies in the pot culture. We have also developed a bioformulation which is talc powder based, for individual bacteria and mixed culture. This formulation was used as seed treatment, soil application, seedling root dip and foliar spray in groundnut crop in vitro germination conditions. A. brasilense was found to enhance the tap root growth and P. fluorescens, the lateral root growth. The other growth parameters like shoot growth, number of leaves were enhanced by the combination of both of the bacteria than their individual formulations. Among the method of application tested in our study, soil application was found to be the best in yielding better results of plant growth promotion. © 2017, Academia Brasileira de Ciencias. All rights reserved.

Anais da Academia Brasileira de Ciências

Compatibility of Azospirillum brasilense and Pseudomonas fluorescens in growth promotion of groundnut ( Arachis hypogea L.)

Many plant growth promoting rhizobacteria (PGPR), biocontrol agents and biofertilizer bacteria are used in agriculture. They are applied to crops by seed or soil application which colonizes in the rhizosphere. Although there are extensive reports on the output (growth, yield, disease resistance, phenotypes, etc) of the combined inoculations of these bacteria the companionship among these bacteria and the relative contribution to overall output is largely unknown. The starting point to unravel this would be to study the bacterial companionship in terms of ability to co-aggregate, stability of co-aggregation and developing molecular evidences for co-colonization in crop roots. Our work mainly aimed at understanding the extent of co-aggregation between Pseudomonas fluorescens and Bacillus subtilis and their co-colonization abilities in plant roots. This was verified by conducting several experiments of co-aggregation and effect of various factors like pH and growth phase, effect of various treatments like urea, protease K, EDTA, heat, thermal stability, sonication and desiccation on the extent of the co-aggregation. Co-aggregation between these two bacteria was found to be pH and growth phase dependent. Protease and EDTA treatment reduced the co-aggregation whereas sonication treatment improved the same. Talc based formulation of the bacteria was prepared separately and used for seed treatment in black gram. The seeds were germinated under laboratory conditions and growth parameters were observed. DNA from roots of germinated seedlings was used as template to amplify bacterial DNA with species specific primers. PCR analysis results indicated that P. fluorescens and B. subtilis are compatible in colonizing black gram. © 2015 Elsevier Ltd.

Biocatalysis and Agricultural Biotechnology

Co-aggregation of Pseudomonas fluorescens and Bacillus subtilis in culture and co-colonization in black gram (Vigna mungo L.) roots

Gaming Law Review and Economics

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Journal of Agricultural and Food Chemistry

Isolation and Characterization of Leu7-Surfactin from the Endophytic Bacterium Bacillus mojavensis RRC 101, a Biocontrol Agent for Fusarium verticillioides

FEMS Microbiology Ecology

Molecular-based strategies to exploit Pseudomonas biocontrol strains for environmental biotechnology applications

Journal of Bacteriology

Genetic Characterization of Pseudomonas fluorescens SBW25 rsp Gene Expression in the Phytosphere and In Vitro

Microbiology and Molecular Biology Reviews

Unraveling the Secret Lives of Bacteria: Use of In Vivo Expression Technology and Differential Fluorescence Induction Promoter Traps as Tools for Exploring Niche-Specific Gene Expression

Pseudomonas fluorescens-mediated biocontrol in the post-genomic era: From lap to lab to land

Journal	Data powered by TypesetBiotechnology Journal
Publisher	Data powered by TypesetWiley
ISSN	1860-6768
Open Access	No