Bioremediation is an essential feature of microorganisms concerning contaminations in soil and water. The use of microorganisms has been proved to be an effective treatment of industrially released effluents comprising of heavy metals, such as chromium (VI). In the current study, seasonal variations were observed in the concentrations of chromium(VI) as the samples from selected locations showed an increase in mean concentration during the summer compared to the low mean during winter, suggesting excessive evaporation in the summer leading to the heavy metal accumulation. Among the 35 isolates obtained from tannery effluent contaminated wastewater sources the 3 unique strains identified as Streptococcus pyogenes strain APRRJVITS10, Pseudomonas putida strain APRRJVITS11, and Bacillus thuringiensis strain APRRJVITS15, showed tolerance toward chromium(VI) and the maximum tolerance for each strain was 1250 ppm. The media optimization through shake flask methods showed chromium(VI) (in 100 ml LB broth) removal of 47.82%, 48.11%, and 49.93% by S. pyogenes, P. putida, and B. thuringiensis respectively. Further, Pseudomonas putida showed chromium(VI) (in 1500 ml LB broth) removal of 50.48% in optimized conditions, proving to be highly potential for treating effluent wastewater for chromium(VI) removal. © 2021 Taylor & Francis Group, LLC.