The emergence of modern microbial technology and product biotechnology had significantly made over the way, scientists and researchers view differently the microbes and the product they biosynthesize. Polymers create a significant role inside the natural environment and also play a vital action in the fashionable industrial economics. Some of the natural biopolymers like proteins and nucleic acids pass the biological information both in the intracellular and extracellular environment. Other polymers such as sugar based polysaccharides provide metabolic energy for the cellular activity and play important role with their potential applications in the different sectors. For instance, those can be used as lubricants, absorbents, adhesives, cosmetics, drug delivery, structural materials, lithography and for the manufacturing of computational switching devices). The main constraints and major obstacles for the full commercialization are due to their production cost, which is related to cost of the substrates and downstream processing. In this research study purification and invitroanalysis of pullulan will be discussed.