Influence of reinforcement effect on mechanical properties of banana bio fiber polyester has been investigated. Three different type of weaving patterns such as plain, basket and twill banana fabric were used to analyze the mechanical properties of composites. Results revealed that composite with basket weaving style enhances the properties of composite material compared to other two patterns. Further, same weight percentage of banana fiber was reinforced in the polyester matrix with random orientation. Three different lengths (3 mm, 4mm and 5 mm) have been employed to analyze the influence of random orientation of composites mechanical properties compared to woven reinforcement. Results depicted that composite with woven reinforcement enhances the properties of composites compared to short fiber with random orientation. Reason is woven fiber reinforcement enhances the young's modulus of composite laminate which offers more resistance against deformation and transfer stress uniformly from reinforcement to matrix. © 2017 IAEME Publication.