Reverse Vaccinology (RV) combines bioinformatics and biotechnological techniques to develop a vaccine. It involves initial screening of the whole genome for the prediction of possible antigens which includes extensive in vitro analysis leading to the development of a new vaccine. This technique has reduced considerable amount of work, time and cost in involved in the development of a vaccine to a greater extent. The review analysis the application of RV in major bacterial pathogenic diseases. The availability of whole genome sequences of many human pathogens, RV could be implemented to develop a much better vaccine covering almost all the possible strains and serotypes of each pathogen. However, RV is lacking in the development of vaccines against viral infections, though their whole genome sequences are available.