Pullulan is a natural water-soluble exopolysaccharide, produced from starch by fermentation. Pullulan is used as a glazing agent, as a film-forming agent, as a thickener or as a carrier in the production of capsules for dietary supplements, as a substitute for gelatin, as a texturizer for chewing gum, coatings for coated tablets containing dietary supplements, for production of edible flavoured films, used as breath fresheners, in the production of jams, confectionery, some meat and fruit products. Pullulan consists of maltotriose units linked through a 1, 6 - glycosidic bond. Screening of pullulan producing microorganisms has gained importance nowadays to obtain pullulan which is known to have a wide range applications in medicine, food industry, pharmaceutical, electronic and cosmetic companies. In our study we screened Aureobasidium pullulons, a Pullulan producing microorganism from Leaves of Mangifera indica, saraca indica, Ficus bengalensis, Hibiscus rosa sinensis, Nerium sp., Tamarindus indica leaves from a village named Iruvaram in chittoor district of Andhra Pradesh and are used for the isolation of Aureobasidium pullulons. The organism (Aureobasidium pullulons) was obtained from the leaves of Mangifera indica and Ficus bengalensis. The color variant isolate obtained from the leaves of PICKS bengalensis was named SSW and the pink colored colonies obtained from leaves of Mangifera indica was named SSP respectively. The organism obtained was identified and characterized by morphological identification through microscopic study. Screening methodologies, microscopic examination will be discussed. © Global Science Publications.