Spectacles are used by millions of people every day. Although it is an item of daily use for many, not all feel the need to clean their glasses daily [1] . As, most of today’s lenses in use are made of polycarbonate, this experiment aims at studying the various organisms present on polycarbonate lenses. From staining, Biochemical assay tests and other analytical techniques, different species of bacteria are noted and finally identified. Shigella spp. were identified as the major microbial contanimant on these polycarbonate lenses. Shigella most commonly causes shigellosis and dysentry in humans [2] . © RJPT All right reserved.