Pectinase is an inducible and extra cellular enzyme among those Pectin Lyase (PL) is a industrially exploited enzyme which cleaves α-(1,4)- glycosidic linkage by trans elimination which results in galactouronidine with double bond between C-4 and C-5 at non reducing sugar ends. In our study we have screened Pectin lyase producing Actinomycetes or Actinobacteria from fruit industrial waste enriched soil samples collected from Katpadi and Chittoor. Many industrially enzymes needs presence of certain inducible and non protein substrates. In the case of pectinase even though it is a cock tail enzyme but it needs same substrate, i.e. Water soluble colloidal high molecular weight carbohydrate normally present in ripened fruits i.e. pectin. Its estimation in some fruits and vegetables indicates the range of 0.6-30. mg/mL and the cheapest source for pectin substrate. Among 100 soil samples two strains of Actinomycetes were exhibiting 52-65 PL/mL activity. Degradation of pectin were studied and degradable products were analyzed and Future studies on purification, Strain improvement and statistical analysis is carried out. © Global Science Publications.