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Screening, biochemical and molecular identification of novel streptomyces fumigatiscleroticus VIT-SP4 derived cocktail pectinase from border industrial pectin enriched places of Andhra Pradesh and Tamil Nadu
G. Praveen Kumar,
Published in Research Journal of BioTechnology
2017
Volume: 12
   
Issue: 4
Pages: 22 - 29
Abstract
The present study is concerned about the isolation of novel cocktail pectinolytic actinomycete from different pectin-rich fruit industrial residue dump sites in the border places Gudiapala, Naraharipet of Andhra Pradesh and Kangeyanellur, Mettukulam of Tamil Nadu States of India. Hundred soil samples were screened by primary and selective enrichment procedures. Isolates were analyzed for pectin lyase (PL), polygalacturonase (PG) and pectin esterase (PE) activity. The strain (designated as VIT-SP4) was able to produce maximum pectinase of solid state fermentation (pectin substrate orange peel was degraded within 72 h) and was taken for further study. The potential strain was identified by morphological, biochemical and molecular characterization. The results revealed that the potential strain was Streptomyces fumigatiscleroticus VIT-SP4 based on sequencing of nucleotide sequence of 16S rRNA gene. The sequence was successfully submitted to Nucleotide sequence database (GenBank).The novel natural-dye assisted calcium alginate immobilization technique has been investigated for crude pectinase for long term usage. The study revealed the better stability and reusability of immobilized pectinase in comparison with free pectinase. Streptomyces fumigatiscleroticus VIT-SP4 strain could be potential strain for cocktail pectinase production commercially in industrial field in mere future.
About the journal
JournalResearch Journal of Biotechnology
PublisherResearch Journal of BioTechnology
ISSN09736263