The main focus of the present investigation was to screen the bioactivity of Streptomyces sp VITJS3 isolated from Bay of Bengal, Puducherry coast of India. The ethyl acetate crude extract checked for antibacterial activity showed better zone of inhibition at 20mg/ml concentration against 5 pathogenic bacteria Staphylococcus aureus (23mm), Pseudomonas aeruginosa (23 mm), Escherichia coli (11 mm) and Salmonella typhi (22 mm ). The antifungal activity of the crude extract showed moderate zone of inhibition at 20mg/ml concentration against Aspergillus niger (14mm), Aspergillus flavus(11mm), Aspergillus fumigatus(15mm) and Fusarium oxysporum(12mm). The antioxidant potential of the crude extract evaluvated at 1000, 500, 250, 125, 62.5 and 31.75 μg/ml concentrations exhibited strong reducing power activity with 90% percent inhibition. The GC-MS profile of the JS3 crude extracts revealed the presence of volatile compounds. The morphological, physiological, biochemical properties of the strain VITJS3 was identified and confirmed as Streptomyces sp. Thus the study evidence the strain Streptomyces sp VITJS3 has various bioactive potential and hence further studies on purification and structure elucidation of the lead molecule will ensure the active compound for therapeutic use.