Azoospermic patients are reported to have either complete or partial deletions of USP9Y gene ecoding Ubiquitin specific peptidase 9, Y-linked (USP9Y) enzyme, located on the q arm of Y chromosome. The gene is known to be involved in normal spermatogenesis; however, its function is not well understood. In this study, we amplified a specific region of exon-12 of USP9Y gene from 22 azoospermic patients using polymerase chain reaction and then PCR products were screened for polymorphism using single strand conformation polymorphism (SSCP). SSCP analysis showed band variation in one azoospermic patient. © 2014, Sphinx Knowledge House. All rights reserved.