The objective of the study is to identify a potent streptokinase producing streptococcus sp. from different samples. Various food samples and soil samples were collected and screened for potent streptokinase producing Streptococcus strains. Isolates were confirmed as Streptococcus sp. based on its morphological and biochemical characterization. Further they were screened for the streptokinase activity. Isolates from curd sample and bore soil samples showed good activity and they were taken for further analysis and production process. They were compared based on their activity of clot lysis and casein digestion. Isolate from curd sample showed partial hemolysis and the isolate from bore soil sample showed complete hemolysis on blood agar medium. Production of streptokinase from these Streptococcal strains were done and partially purified. © RJPT All right reserved.