Objective: screening the antibacterial and antioxidant activity of actinomycetes isolated from three different garden soil samples of VIT University, Vellore, Tamil Nadu, India. Methods: All the isolates were evaluated for their inhibitory activities against four clinical pathogens by agar well diffusion method and assessed for its antioxidant potential using DPPH scavenging assay. The presence of volatile compounds in crude extract was analyzed by Gas chromatography-mass spectrometry (GC-MS) analysis. Results: It was observed that one strain AC 23 showed broad spectrum activity at the concentration of 30mg/ml against Bacillus cereus (35mm), Staphylococcus aureus (38mm), Pseudomonas aeruginosa (19mm), Escherichia coli (40mm), Salmonella typhi (22mm). The antioxidant potential of the crude extract was found maximum at the concentration of 20mg/ml with 60% inhibition. Further the cultural characteristics of the strain AC23 was studied on different culture media. The 16srRNA gene sequence of the strain AC23 revealed the degree of sequence similarity 98% with Streptomyces werraensis and it was validated by existing taxonomic reports. Conclusion: These findings may have capability to identify some of the most important lead compounds ever developed.