Enzymes are used in industries for variety of purposes. Microbial keratinase enzyme offer a substantial advantage of degrading keratin waste. In large amount of keratin are present in the soil, nail, hairs, feather and poultry. In many microorganisms are secreted keratinase degrading enzyme used in our study were isolated from poultry waste soil and feathers. The isolates were obtained from culture filtrate was measured in units by keratinolytic activity and their activity was measured used by different incubation periods pH, temperature and different agitation. The highest enzyme activity was observed at 50°C and pH 8 .The enzyme was stable at 40 to 50°C. It was found to be highly stable within the range of 5- 8.5 pH values. Complete feather degradation was achieved at pH 8, inoculum size 2%, agitation rate 200 rpm, and incubation temperature 37°C, incubation period for 48 h. Feather keratin is highly resistant to degradation, but some keratinase produced microorganisms can easily degraded insoluble keratins. These keratinase produced species have an important application in removal of poultry waste and recycled into valuable by product. Maximum feather degrading activity attained at the optimal conditions. In practical applications, keratinase is a useful enzyme for promoting the hydrolysis of feather keratin and improving the digestibility of feather meal. The aim of this study to identify new keratinolytic bacteria and its optimum parameters for higher feather degradation at pH 8 and 500C, with potential application in biotechnological processes.