This study was performed to screen for an α-amylase producing isolate from soil. The enriched soil samples were collected from various rice mills surrounding places around the town of Vellore. Microorganisms were isolated using baiting plate method using various fruits and vegetable pieces like carrot, tomato, potato, pumpkin and cabbage. After 24 hour incubation, the plates were observed and three colonies were isolated and sub-cultured by streaking. The amylase activity was checked by spread plate method using 1% starch in LB agar. The colony that was showing high amylase activity was then subjected to Gram staining and spore staining. The microbial growth was tested at various temperatures and pH to specify its growth limitation. The growth curve of this microorganism was plotted as a function of OD at various time intervals, checked by a colorimeter at 600nm. Finally, the biochemical testing was done for further characterization of the amylase producing microorganism.