In this study, we demonstrate that a co-culture approach between C2C12 myoblasts and pheochromocytoma PC12 neuronal cells allows for better formation of muscle-like tissues than a mono-culture of C2C12 cells. In particular, we used a micromolding technique to fabricate microgrooved patterns of methacrylated gelatin (GelMA) hydrogel, which served as a substrate to culture encapsulated cells in GelMA and to induce myoblasts alignment. GelMA is a semi-natural polymer that is easy to pattern by UV irradiation for making 3D structures in which cells can proliferate. Myoblasts and the resulting myotubes were analyzed in terms of alignment and length, respectively, in the mono-culture and the co-culture systems for both patterned and unpatterned regions.