Hydroxyapatite (HA) nanostructures may be an advanced candidate in biomedical applications for an apatite substitute of bone and teeth than other form of HA. In contrast, well-defined size and shape control in synthesizing HA nanostructures is always difficult. Solvothermal synthesis method was adopted to prepare highly ordered hydroxyapatite nanostructures. The morphological analysis by FESEM confirms that the preparation conditions greatly influences the morphological characterization. X-ray Diffraction (XRD) measurements indicate the formation of crystalline hexagonal hydroxyapatite. The crystillinity of HA decreased with increasing ethyline glycol. © (2013) Trans Tech Publications, Switzerland.