The use of castellated beams has increased a lot in the past few years in all types of structures. The castellated beam is one of the several methods used to decrease the weight and cost of steel used in the construction. In castellated beams, increased depths can be achieved without increasing the self-weight of the structure. The significant advantages of castellated beams are better possessing bending moment capacity, ease of providing service pipes and wires, and aesthetic appearances. The castellated beams are made by making a zigzag cut through the web according to the desired opening shape and size, and then the two parts are welded together. In this work, static and dynamic analyses of the castellated steel beam section (ISMB 300) are performed to compare deflection, stress distribution, shear Stress, amplitude, and natural frequency. The investigation is done using ANSYS 18. The Castellated beam having a maximum opening of size 0.6h is used for the study. The castellated beams have proved to be useful under a light-uniform load. © 2021 Institute of Physics Publishing. All rights reserved.