Statistics and Mathematical models can be in many forms, including differential equations, or theoretic models. Lack of correlation between theoretical mathematical models and experimental measurements sometime leads to development of better theories in the area of biology. The mathematical design finds wide application in nutrient media optimization for microbial enzyme production. On the other hand these mathematical tools in the field of statistics play an important role with data analysis of proteomics and genomics studies. For the MS/MS spectroscopic data, micro array, RT-PCR data analysis, linkage study, gene-gene interaction studies are very difficult to interpret, but the statistical tools made it very easier from the conventional method of data analysis in both Biotechnology and Bioinformatics. © 2013 Suneetha V. et al.