Lipase enzymes are currently attracting the most considerable amount of attention due to their unique characteristics. The isolate Pseudomonas sp. VITSDVM1was mutated using chemical mutagens: Ethyl Methyl Sulfonate (EMS), Ethidium bromide (EtBr), Sodium azide and N-methyl-N'-nitro-N-nitrosoguanidine (NTG), in order to enhance its production and lipolytic potential. During the treatment, mutants were qualitatively and quantitatively selected. The results revealed that EMS was found to be the best inducer which showed maximum lipase production (4.96U mL-1). This mutant showed an overall increase in activity over its parent strain for the production of extracellular lipase. Optimisation parameters for the lipase activity was found maximal at pH 8 and at 37oC, utilizing glucose and yeast extract as carbon and nitrogen sources. The study indicated that the enzyme activity of the mutant strain using chemical mutagen was 2-fold higher than the wild strain. The partially purified lipase enzyme was analysed by high-performance liquid chromatography, revealed its RT at 3.065min. Based on the findings of present study Pseudomonas sp. is an ideal candidate for extracellular lipase production for industrial uses and it can be exploited in various purposes.