Electrocardiogram (ECG) is a biological signal that plays a significant role in the detection of heart diseases. Translating the QRS complex is a standout amongst the most imperative parts of ECG signals and its processing and flag preparing. R wave is the real segment in the intricate, which has a basic job in the analysis of heart Rate abnormalities and furthermore in deciding pulse fluctuation (HRV). In this paper, we are utilizing MATLAB to pin the information from the crude MAT-documents from physio bank and Kubios HRV programming to dissect the information. Three unique methodologies were taken for consideration for the investigation, Time-area analysis, Frequency space analysis, and non-linear analysis methods. The correct utilization of MATLAB capacities (both implicit and user characterized), toolbox and Simulink software can lead us to work with raw ECG signals for preparing, separating and examine by simulation with extraordinary accuracy and ease. The product acknowledges distinctive info information groups for electrocardiogram (ECG) information and beat-to-beat RR interim information. The product figures all the normally utilized time-area and recurrence space HRV parameters and more than 40 investigation parameters. The examination results are spared as an ASCII content record, MATLAB MAT-document, or as a PDF report. The product is essentially a direct result of its reduced graphical UI. The product can be utilized uninhibitedly on Windows and Linux working frameworks.