Co-immobilization of Urease (Urs) plus glutamate dehydrogenase (GLDH) over titania-zirconia (TiO2- ZrO2) nanocomposite and its chemical reactions was examined. TiO2-ZrO2 nanocomposite was incorporated utilizing sol-gel spin covering method with blend of Zirconium (IV) n-propoxide, titanium (IV) butoxide in 1:1 molar proportion. The electrochemical investigations used for urea discovery containing reference (Ag/AgCl), counter (ITO) as well as operational (Urs-GLDH/TiO2-ZrO2/ITO) sensing matrix and the modification of bio electrode is finished by physical adsorption procedure. This enzymatic urea sensor indicates linearity as 5- 100 mg/dL with enhanced affectability as 2.64 μA [Log mM]-1 cm-2 and lower discovery boundary as 0.08 mg/dl (0.42 mM). The XRD of TiO2- ZrO2 nanocomposite demonstrates crests at 2θ:30.7° and showing the nearness of prevalent orthorhombic period of ZrTiO4. The band found at 3520 cm-1 and 1585 cm-1 relate to extending tremor as well as twisting of O-H bond because of assimilating water atoms and cyclic voltammetric (CV) also made foe the electrochemical analysis of uric acid in the analyte. The sensitivity is based on the immobilization of enzymes over the matrix surface. This is credited to the great electrocatalytic conduct of the nanocomposite based uric acid biosensor. © 2019 IEEE.