Being the natural organic component in bone, collagen extracted from collagenous tissues is used in the development of matrix for bone regeneration. In the present study, an effort has been made to screen the collagen from skin of stingray fish which is naturally calcified for the purpose. Soluble collagen was extracted from the skin of stingray fish and stabilized with glutaraldehyde and gambier. The stabilized collagen was used as matrix for hydroxyapatite growth by alternate immersion cycles in 1 M Tris-HCl buffer containing 200 mM calcium chloride and 120 mM disodium hydrogen phosphate buffer. The composites were characterized with respect to calcification efficacy, hydrothermal stability and biodegradability. The results show that the calcification was higher for stingray skin (SRS) collagen than that for rat tail tendon (RTT) collagen with both the cross linking agents. Glutaraldehyde cross linked SRS collagen had a higher shrinkage temperature and was more resistant to bacterial collagenase degradation than gambier treated one. The study reveals that glutaraldehyde stabilized SRS collagen may serve to be an ideal candidate to be used for developing scaffold for regeneration of hard tissues. © 2014, The Korean Tissue Engineering and Regenerative Medicine Society and Springer Science+Business Media Dordrecht.