The nano-polymeric pharmaceutical excipient such as chitosan nanoparticles (CS-NPs) were synthesized for formulating the anticancer drug Sunitinib (STB). The formulation was done through the simple ionic cross linking method. The prepared formulation was characterized by DLS, SEM, FT-IR and XRD. The DLS study reveals that the Sunitinib loaded chitosan nanoparticles (SNB-CS-NPs) were in the size range of < 200 nm. Encapsulation of Sunitinib and validation for the formulation was done using UV spectrophotometry. In vitro drug release and its cytotoxic studies were performed for STB-CS-NPs. This study implies the novel drug delivery system for Sunitinib for the effective sustained delivery.

Sangeetha D

Department of Chemistry

School of Advanced Sciences

Vellore Campus

Joseph J.J

Gomathi T.

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

International Journal of Biological Macromolecules

Abstract: The present study involves the preparation, characterization, and evaluation of novel alginate nanocarrier for sunitinib (STB), an anticancer drug. Sunitinib (STB) is a weakly soluble drug in water due to its poor dissolution rate and oral bioavailability. Alginate biopolymer is one of the promising candidates for a delivery matrix. A controlled gelification method using calcium chloride as the crosslinker was done for the formulation of STB using nanoalginate. The objective of this work was to evaluate the physicochemical/biological properties of the release system via in vitro dissolution studies and cytotoxicity studies. The bare alginate nanoparticles and STB-loaded alginate nanoparticles were characterized for its physicochemical properties using FT-IR, SEM, and TEM studies. The formulation and loading chemistry were well exhibited by FTIR analysis results. SEM and TEM results revealed the spherical and rod-like shape morphology with rough surface where the drug molecules were adhered. The entrapment efficiency and dissolution studies of the drug were done through UV VIS instrument. In vitro cytotoxic studies were performed for loaded sunitinib alginate biopolymer (STB-AL-NPs). The cytotoxicity results reveal that the cytotoxicity is mainly due to the loaded anticancer drug sunitinib. Lay Summary: In this study, sunitinib drug was encapsulated in alginate nanoparticles effectively. The interaction between polymer, crosslinker, and drug was well determined using FTIR, XRD, SEM, TEM, and Zeta potential. The results are compared with bare alginate nanoparticles. The expected entrapment efficiency of the drug carrier was around 99% via UV-Vis spectroscopy, proving good drug-polymer interaction. In vitro drug release studied was done by dissolution method using 900 mL of 0.2 mM PBS, to determine the sustained release of the drug from the excipient. The results proved that the drug was released to the medium in the sustained manner. Future Scope: Sunitinib-encapsulated alginate nanoparticles not only offer several advantages over conventional drug therapies but also expected to overcome the side effect regarding to dosing and toxicity while administration. However, further optimization studies includes stabilization and targeting should be performed for both in vitro and in vivo.

Regenerative Engineering and Translational Medicine

In vitro Release and Cytotoxic Studies of Novel Alginate Nanocarrier for the Antitumor Drug: Sunitinib

Polysaccharides were extracted from the dried fruiting bodies of Pithecellobium dulce with 20% ethanol by microwave-assisted extraction. The polysaccharides were isolated by ion exchange chromatography and afford three water-soluble polysaccharides PDP-1, PDP-2, and PDP-3. These isolated compounds were subjected to acid hydrolysis, methylation, IR and GC-MS for its compositional analysis and revealed that all the three fractions are heteropolysaccharides. PDP-1 was found to be composed of xylose, mannose, galactose and Rhamnose. PDP-2 and PDP-3 composed of xylose, Rhamnose, glucose, ribose, galactose, and mannose. The micromeretic properties of the extracted polysaccharides possessed a bulk density of 0.69g/ml, 0.65g/ml and 0.71g/ml for PDP-1, PDP-2, and PDP-3 respectively. The Hausner's ratio and Carr's index confirm the good flow property and compressibility of the polysaccharides. The polysaccharides extracted from Pithecellobium dulce fruits were tested for its application as a pharmaceutical adjuvant. The in vitro drug release study suggests that the extracted polysaccharides are potential candidates as a pharmaceutical adjuvant. Furthermore, the three isolated polysaccharides were subjected to its radical scavenging activity using DPPH, phospho molybdenum assay and reducing power assay. The results exhibited that the polysaccharides can be explored as a novel natural antioxidant and can be recommended as a functional food.

Screening of natural polysaccharides extracted from the fruits of Pithecellobium dulce as a pharmaceutical adjuvant

International Journal of Pharmaceutics

Studies on drug-polymer interaction, in vitro release and cytotoxicity from chitosan particles excipient

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Factorial design as tool in chitosan nanoparticles development by ionic gelation technique

Journal of Controlled Release

Chitosan-based siRNA delivery systems

Food Chemistry

Chitosan–tripolyphosphate submicron particles as the carrier of entrapped rutin

Journal of Biomaterials Science, Polymer Edition

Curcumin-LoadedN,O-Carboxymethyl Chitosan Nanoparticles for Cancer Drug Delivery

Sunitinib loaded chitosan nanoparticles formulation and its evaluation

Journal	Data powered by TypesetInternational Journal of Biological Macromolecules
Publisher	Data powered by TypesetElsevier BV
ISSN	0141-8130
Open Access	0