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Susceptibility Testing of Methicillin ResistantStaphylococcus aureus(MRSA) and Biological Role of Silver Nanoparticles of Honey Against MRSA
Singh Nidhi,
Published in Informa UK Limited
Volume: 4
Issue: 5-6
Pages: 332 - 342

Methicillin resistant Staphylococcus aureus (MRSA) is a very common nosocomial pathogen and in this study the occurrence of MRSA infections and their susceptibility pattern were tested. A total of 50 MRSA strains were collected from pus samples. All isolates were found to be sensitive to vancomycin and imipenem. Further minimum inhibitory concentration (MIC) of vancomycin was performed by agar dilution method, 24 % of the strains were inhibited at a concentration of 4 µg/ml and other 10 % at 8 µg/ml. It was observed that none of the isolates could tolerate 16 µg/ml and E-test also showed that 64 % of them were sensitive and 36 % were intermediately resistant. All the strains were tested for slime production by Congo red agar method and biofilm formation by microtitre plate method. Out of the total strains tested 58 % exhibited positive result for slime production and 70 % were biofilm positive. All the MRSA strains were tested for MIC with two types of honey which gave MIC of 30 %. Sarvodaya honey and Tulsi honey were chosen for the study. Further silver nanoparticles were synthesized using honey and the antimicrobial activities against the pathogens were checked. The study showed that honey has antibacterial activity against test organisms and may form an alternative therapy against certain bacteria. © 2014, © 2014, Har Krishan Bhalla & Sons.

About the journal
JournalJournal of Biologically Active Products from Nature
PublisherInforma UK Limited
Open Access0