The traditional System of medicine comprises a wide variety of formulations for various ailments. It is essential to Standardize Ayurvedic drugs so that drugs having good quality can be used for better effect. Standardization is not only limited to the final preparation, but it also includes the materials used during the development and SOP adopted and the equipment used. The present study is an attempt to prepare anti anaemic herbomineral nanoceuti-cal by two different processes, and to standardize and evaluate the haemopoi-etic effect of the prepared formulations and compare with a marketed formu-lation. Preparation was done based on Ayurveda Pharmacopoeia. Formulations were characterized by conventional methods and by AAS, XRD, EDAX, SEM, TGA, and IR. The toxicity and anti-anaemic effectiveness are evaluated by using albino Wister rats and histopathology and cytotoxicity studies. Products have a crystalline nature (XRD). TGA shows the decomposition temp of ferric oxide. SEM EDAX revealed surface smoothness and nano to the micro size of particles. AAS shows that the elements are within the prescribed limits. The in-process Standardization shows the effectiveness of adjuvants. There is an increase of haemoglobin and body weight on day 75 for first preparation than the Second and were comparable to that of standard. No toxicity in all rats and indicated no renal and gastrointestinal toxicity. The size, smoother sur-face, and the therapeutic effectiveness of the herbominerals prepared by the two methods can be attributed to the calcination steps and adjuvants. Thus, maybe dose reduction and rapid effect can be achieved. © 2020 International Journal of Research in Pharmaceutical Sciences. All rights reserved.