A novel polyamide first-generation dendrimer 7 was synthesized from new methodology under mild reaction conditions. The AB2 adduct, ethyl 2-[N-[2,2-bis(hydroxymethyl)propionyl]amino]ethylate (3), was prepared from 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) and glycine as a linker. Further, G1 dendrimer 7 was synthesized by coupling melamine (5) with monomer 3. The structural elucidations of all the compounds were confirmed by FTIR, 1H & 13C NMR and HRMS. The G1 dendrimer was found to exhibit significant antibacterial activity against S. aureus and E. cloacae and antioxidant activity by DPPH scavenging method when compared to the standard, ascorbic acid. © This is an open access journal, and articles are distributed under the terms of the Attribution 4.0 International (CC BY 4.0) License. This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit the author for the original creation. You must give appropriate credit, provide a link to the license, and indicate if changes were made.