Many male infertility cases are because of genetic and environmental factors and most of them are idiopathic. About 10-20% of azoospermic patients are showing the microdeletion in Y-chromosome. The azoospermia factor or AZF region at the Yq11 position which consists of genes those are necessary for spermatogenesis. In Y chromosome microdeletion, deletion in AZFa region is very rare. The aim of our study is to find out the frequency of microdeletions in Y chromosome particularly in AZFa region in azoospermic men of Vellore cohort. For this study, we collected 10 azoospermic patient and 10 control men samples from the Sandhya hospital, Vellore. In this study, we mainly focused on AZFa region to analyze the frequency of microdeletions in Y chromosome using SY82 (264bp) and SY83 (275bp) STS markers. There was an absence of microdeletion in patient samples for SY82 and SY83 markers of AZFa region. We concluded that sample size should be increased to confirm our results. © Published under licence by IOP Publishing Ltd.