An improvisation in the heat exchange equipment has become more vital nowadays, due to the increase in cost of energy and material existence. The rate of heat transfer will enhance by causing a change in the flow of fluid by breaking the thermal and viscous boundary layers, also by introducing the nanofluid. The main idea of this paper is to evaluate the friction factor (f), change in pressure drop (ΔP), outlet temperature (Tout), and thermal performance (Æž) of base fluid alone (DI Water) and base fluid with nanofluid (Al2O3) flowing in a tube with the insert (twisted tape) with holes of different pitch ratio(Y=6, Y=4), at fixed heat flux value in laminar and turbulent flow condition. The introduction of different pitch ratio(Y=6, Y=4) in modified tube with nanofluids to additionally improve the heat transfer and outlet temperature of the working fluid is a new technique. For Laminar flow condition with DI water as fluid & twisted tape insert of Y=6 & Y=4, the increase in average outlet temperature & Pressure drop is 4.06 %, 0.7 % and 0.07 N/m2, 0.11 N/m2, however for Al2O3 nanofluid & with twisted tape insert of Y=6 & Y=4 the increase in average outlet temperature & Pressure drop is 9.01 %, 8.4 % and 0.10 N/m2, 0.11 N/m2. Similarly for turbulent flow conditions with DI water as fluid & twisted tape insert of Y=6 & Y=4, the increase in average outlet temperature & Pressure drop is 2.3 %, 2.1 % and 3.9 N/m2, 4.6 N/m2, however for Al2O3 nanofluid & with twisted tape insert of Y=6 & Y=4 the increase in average outlet temperature & Pressure drop is 4.8 %, 8.6 % and 5.3 N/m2, 6.3 N/m2 © 2019 Elsevier Ltd.