Sweet Flag (Acorus calamus L.) leaf and rhizome tissues were analyzed for biochemical compositions notably of carbohydrates and lipids. The glycolipid content measured in rhizome tissue was 62.3mg%/FW almost double the glycolipid content (28.8 mg%/FW) in leaf tissues, whereas the sterol content in the leaf tissue (47.9 mg%/FW) was three times of the sterol content in rhizome tissues (15.5 mg%/FW). Carbohydrates content such as total sugar, reducing sugar, sucrose and fructose measured in leaf and rhizome tissues were more or less similar, with slightly higher values of total sugar (18.2 mg%/FW) in the leaf tissues. The study thus revealed variation in biochemical compositions in two different tissues leaf and rhizome of A. calamus.