Current report in this study, using plant-derived nanoparticles and green synthesis has become the emerging area of research. It was originated to enhance our insight on the strength and environment impact of silver nanoparticles (Ag-NP). The particles were synthesized using Passiflora caerulea leaf extract and characterized by using UV-visible, XRD, TEM, SEM, EDAX, FTIR, AFM analysis. The silver nanoparticles (AgNPs)are known to be one of the most versatile inorganic nanoparticles with its application in the treatment of human pathogens, and we focused the nanoparticles toxicity. In the result, we found a maximum zone of inhibition Trichophyton rubrum 104.648 ± 0.008 when compared with the control. The mean standard deviation for the silver nanoparticles which was available for the size was nearly 23.94 ± 8.67 nm. Besides, the toxicity was life stage dependent. The zebrafish embryos/larvae are additionally mentioned lethal concentration of silver nanoparticles 80 μg/ml and instructed during this study as a good protocol for evaluating the potential toxicity of nanoparticles. © 2019