Tea is an important agricultural commodity in many Asian, African and South American countries. Tea is cultivated for its tender leaf and any disease of leaf seriously affects the production of tea. Among the leaf diseases, grey blight caused by Pestalotiopsis theae is one of the most important and devastating diseases in tea. This problem persists from a long time, even though genetic improvement strategies are not so successful in tea due to lack of cloned genes from this plant. To overcome this problem, we have attempted this study. An enriched cDNA library during grey blight disease infection in tea has been constructed. The cDNA library contained about 1200 clones. One thousand colonies were picked up and screened by colony PCR to find out colonies with ≥ 250 bp insert. Colony PCR confirmed 480 clones with insert size above 250 bp. Plasmids were extracted from all these colonies and sequenced. 320 sequences were identified as unique. Among these sequences, 67 sequences have a high degree of sequence similarity with genes from other species which were related to disease resistance/stress tolerance. 53 sequences have a high degree of sequence similarity with hypothetical proteins/ribosomal proteins while 200 sequences have no significant match in the database and are considered as novel. The cDNA library specific to grey blight disease infection yielded many important disease resistance genes and genes involving signal transduction pathways in the form of ESTs and partial CDS. Based on the sequence information, these genes can be isolated in full length and utilized in future crop improvement programs like developing increased disease tolerant/resistant varieties. © 2018 World Research Association. All rights reserved.