In this paper, an unsupervised segmentation methodology is proposed for remotely sensed images by using Fractional Differential (FD) based texture analysis model and Iterative Self-Organizing Data Analysis Technique Algorithm (ISODATA). Essentially, image segmentation is used to assign unique class labels to different regions of an image. In this work, it is transformed into texture segmentation by signifying each class label as a unique texture class. The FD based texture analysis model is suggested for texture feature extraction from images and ISODATA is used for segmentation. The proposed methodology was first implemented on artificial target images and then on remote sensing images from Google Earth. The results of the proposed methodology are compared with those of the other texture analysis methods such as LBP (Local Binary Pattern) and NBP (Neighbors based Binary Pattern) by visual inspection as well as using classification measures derived from confusion matrix. It is justified that the proposed methodology outperforms LBP and NBP methods.