The virulence traits of 39 well-defined clinical (29 strains) and environmental (10 strains) isolates of Aeromonas (16 A. hydrophila, 12 A. sobria and 11 A. caviae) were examined by a variety of assays to delineate differences, if any, in the enteropathogenic potential in relation to species and the source of isolation. The distribution of enterotoxin (ent), cytotoxin (cyt) and haemolysin (hae) producing strains of Aeromonas did not correlate to species and source of isolation. The extracellular virulence phenotype Ent+ Cyt+ Hae+ was the most common one among all the three species although unique phenotypes associated prominently with either A. hydrophila or A. sobria were also discernible. None of the cytotoxin or haemolysin producing strains hybridized with the vt1/vt2 or the tdh/trh gene probes, respectively, indicating that these two factors of Aeromonas were distinct. Haemagglutination of human O group erythrocytes was not related to the source of isolation or production of enterotoxin, cytotoxin or haemolysin but appeared to be related to species. The strains which did not exhibit cell-associated haemagglutination belonged to either A. hydrophila or A. caviae. Haemagglutination unaffected by fucose, mannose and galactose was the dominant inhibition pattern exhibited mainly by the clinical haemagglutinating strains of the three species. Only one clinical strain of A. caviae showed a diffuse pattern of adherence to HeLa cells. Expression of the 5 bacterial enzymes by strains of Aeromonas did not fall into a readily discernible pattern in relation to species or source of isolation. From this study, it is clear that the mechanism of the pathogenesis of Aeromonas is a multifactorial one. © 1992, Gustav Fischer Verlag, Stuttgart · New York. All rights reserved.