A sensitive and selective electrochemical sensor for pindolol (PDL) was fabricated by embedding molecularly imprinted polymer (MIP) in graphite carbon paste. PDL imprinted polymer was prepared by free radical polymerization of itaconic acid (IA) and subsequent cross linkage with ethylene glycol dimethacrylate in the presence of PDL. The prepared MIP was subjected to extraction of template and then carbon paste electrode (CPE) was prepared by mixing optimized quantities of graphite, MIP and eicosane. The electrochemical behavior of PDL at MIP and non-imprinted polymer (NIP) modified CPEs have been investigated employing cyclic voltammetry (CV), and adsorptive stripping differential pulse voltammetry (AdSDPV). Chronocoulometry (CC) was used to approximate the surface coverage of analyte molecules which are specifically bound to MIP-CPE. Under the optimal experimental conditions, the sensor displayed linear relationship between the peak current and concentration of the PDL ranging from 0.1 μM to 50 μM, with the detection limit of 0.05 μM (S/N = 3). The presence of most common interferents and other structural analogues of PDL have no deleterious effect on the PDL anodic peak current. The fabricated sensor was successfully applied for the determination of PDL in serum and urine samples with recoveries in between 97 and 105%. © 2016 The Electrochemical Society.