Due to the wide acceptance of White Blood Cells (WBCs) in disease diagnosis, detection and classification of WBC are hot topic. Existing methodologies have some drawbacks such as significant degree of error, higher accuracy, time bound and higher misclassification rate. A WBCs detection and classification called, Jenks Optimized Logistic Convolutional Neural Network (JO-LCNN) method has proposed. Initally, Eulers Principal Axis is used as a convolution model to obtain a rotation invariant form of image by differentiating the background and RBCs, then eliminating them which leaves only the WBCs. By eliminating the wanton features, inherent features are detected contributing to minimum misclassification rate. According to above, Jenks Optimization function is used as a pooling model to obtain feature map for lower resolution. Therefore JO-LCNN is used for removing tiny objects in image and complete nuclei. Finally, Multinomial Logistic classifier is used to classify five types of classes by means of loss function and updating weight according to the loss function, therefore classifying with higher accuracy rate. Using LISC database for WBCs with different parameters as classification accuracy, false positive rate and time complexity are performed. Result shows that JO-LCNN, efficiently improves accuracy with less time, misclassification rate than the state-of-art methods. © 2020 - IOS Press and the authors. All rights reserved.