We have developed a fully printable paperfluidic chip for monitoring oral health.

Ammar Jagirdar

Prasad Shetty

Jagirdar A

S. Satti

Shetty P

S. Garg

Satti S

Debjani Paul

Garg S

Paul D.

Developing countries

Diagnosis

hydrophobicity

Ink jet printers

Oil shale

Olive oil

Printing

Printing machinery

Rural areas

Acidic environment

Buffering capacities

Dental applications

Green techniques

Hydrophobic regions

Patterning techniques

Screening tests

Tooth structure

Printing presses

Dentistry

Diseases

Esterification

Information retrieval

Ink Jet Printing

Water Repellence

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Analytical Methods

We have amplified an 84 bp fragment from the insertion sequence IS6110 of Mycobacterium tuberculosis (MTB) DNA on a paper substrate in 10 min. Tuberculosis (TB) is a highly infectious disease with a high mortality rate in the developing countries. Cheap and rapid screening assays are needed at the point of care for timely intervention and treatment. While PCR-based detection of TB is faster than bacterial culture and more specific compared to microscopy, PCR is not viable as a routine screening test in many low-income countries where TB is endemic due to the requirement of an expensive thermocycler. Disease screening based on isothermal amplification techniques is, therefore, being explored as an alternative to PCR. Successful isothermal amplification of DNA from HIV, H1N1, Chlamydia trachomatis, E. coli, etc. on cheap and disposable paper substrates has been reported in the literature. Isothermal amplification of MTB DNA on paper has been challenging due to the high GC content (65%) of MTB genome. Here we report helicase dependent amplification of a fragment of MTB DNA on a paper substrate in 10 min starting from 100 copies of the template using inexpensive heat sources, such as hot plates and hand warmers. The enzyme mix used to amplify DNA can be spotted and stored dry on paper at ambient temperatures for more than a month. The DNA amplified on paper can be detected by incorporating a suitable fluorescence marker in the reaction mixture or by directly loading the paper in a standard gel electrophoresis set up. Finally, as a surrogate to real clinical sputum samples, MTB DNA was successfully amplified on paper in the viscous environment of artificial sputum. © 2016 The Royal Society of Chemistry.

RSC Advances

Rapid amplification of Mycobacterium tuberculosis DNA on a paper substrate

Indian Journal of Science and Technology

Evaluation of Performance of Cowdung as Microbial Inoculum in Industrial Wastewater Treatment and its Environmental Implications

Lab Chip

Hydrophobic sol–gel channel patterning strategies for paper-based microfluidics

Lab on a Chip

Paper-based microfluidic point-of-care diagnostic devices

Smartphone based health accessory for colorimetric detection of biomarkers in sweat and saliva

Biomicrofluidics

A perspective on paper-based microfluidics: Current status and future trends

A paperfluidic device for dental applications using a novel patterning technique

Journal	Data powered by TypesetAnalytical Methods
Publisher	Data powered by TypesetRoyal Society of Chemistry
ISSN	17599660
Open Access	No