A rapid method to extract DCF from yeast to measure its oxidative status by using a fluorescence spectrophotometer.

Kavitha Thirumurugan

Department of Bio-Sciences

School of Bio Sciences and Technology

Vellore Campus

James J

Fiji N

Debasish Roy

Andrew Mg D

Shihabudeen M.S

Chattopadhyay D

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

&nbsp;

&nbsp;

VIT University

Analytical Methods

Free Radical Biology and Medicine

Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations

Eukaryotic Cell

Involvement of an Alternative Oxidase in Oxidative Stress and Mycelium-to-Yeast Differentiation in Paracoccidioides brasiliensis

Free Radical Research

2′,7′-Dichlorodihydrofluorescein as a fluorescent probe for reactive oxygen species measurement: Forty years of application and controversy

Biomolecular Engineering

Reactive oxygen species and DNA damage after ultrasound exposure

Exposure of Bacillus subtilis to a shear rate of 1,482/s leads to a rapid loss of cell viability after 10 h of growth. Biochemical and molecular evidences provided below strongly suggest that cell death under high shear results from an apoptosis-like process similar to that described in eukaryotes, with activation of a caspase-3-like protease (C3LP) followed by DNA fragmentation. Shear stress leads to an increase in specific intracellular reactive oxygen species (siROS), possibly through activation of NADH oxidase (NOX). The formation of siROS precedes the activation of C3LP and DNA fragmentation, thus establishing siROS as the molecular link between shear stress and apoptosis-like cell death. A model is proposed in which NOX is viewed as being strategically placed on the plasma membrane of B. subtilis that senses and converts a mechanical force arising from shear stress into a chemical signal leading to activation of C3LP, DNA fragmentation, and thus, apoptosis-like cell death. © 2006 Wiley Periodicals, Inc.

Fulltext

Biotechnology and Bioengineering

Reactive oxygen species induced by shear stress mediate cell death in Bacillus subtilis

A rapid method to assess reactive oxygen species in yeast using H2DCF-DA

Journal	Data powered by TypesetAnalytical Methods
Publisher	Data powered by TypesetRoyal Society of Chemistry (RSC)
ISSN	1759-9660
Open Access	0