The use of nanoparticles in food or pharma requires a molecular-level perceptive of how NPs interact with protein corona once exposed to a physiological environment. In this study, the conformational changes of bovine serum albumin (BSA) were investigated in detail when exposed to different concentration of titanium dioxide nanoparticle by various techniques. To analyze the effects of NPs on proteins, the interaction between bovine serum albumin and titanium dioxide nanoparticles at different concentrations were investigated. The interaction, BSA conformations, kinetics, and adsorption were analyzed by dynamic light scattering, Fourier transform infrared spectroscopy and fluorescence quenching. Dynamic light scattering analysis confirms the interaction with major changes in the size of the protein. Fluorescence quenching analysis confirms the side-on or end-on interaction of 1.1 molecules of serum albumin to titanium dioxide nanoparticles. Further, pseudo-second order kinetics was determined with equilibrium contact time of 20 min. The spectroscopic analysis suggests that there is a conformational change both at secondary and tertiary structure levels. A distortion in both α-helix and β-sheets was observed by Fourier transform infrared (FTIR) spectroscopy. Fluorescence quenching analysis confirms the interaction of a molecule of bovine serum albumin to the single TiO2 nanoparticle. Further, pseudo-second order kinetics was determined with equilibrium contact time of 20 min. The data of the present study determines the detailed evaluation of BSA adsorption on TiO2 nanoparticle along with mechanism and adsorption kinetics. © 2016 Elsevier B.V.

Ramalingam C

Department of Biotechnology

School of Bio Sciences and Technology

Vellore Campus

Ranjan S

Dasgupta N

Vellore Institute of Technology (VIT) is a private university located in&nbsp;Tamil Nadu, India. Founded in 1984, as Vellore Engineering College, the institution offers 20 undergraduate, 34 postgraduate, four integrated and four research programs. It has campuses in Vellore, Amravati, Bhopal and Chennai.

VIT is one of the top ranked private universities in India according to NIRF, THE and QS Rankings.&nbsp;Govt. of India has recognized&nbsp;VIT, Vellore as an&nbsp;Institution of Eminence. This has allowed VIT to take independent quality initiatives and move up in world ranking.

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VIT University

Journal of Photochemistry and Photobiology B: Biology

Fulltext

Biotechnology & Biotechnological Equipment

Nanotechnology towards prevention of anaemia and osteoporosis: from concept to market

Due to their small size and unique physico-chemical characteristics, nanomaterials have gained importance in the agri-food sector, notably in preservation and packaging. Future applications will focus on shelf life, food quality, safety, fortification and biosensors for contaminated or spoiled food, irrigating water and drinking water. Different types and shapes of nanomaterials are being used depending upon the needs and nature of the work in agriculture and water quality management. Here we review the application of nanotechnology in agriculture. The major points discussed are: (1) Nanomaterials for agriculture and water quality management. (2) Research interests such as nanoscale carriers, fabricated xylem vessels, nanolignocellulosic materials, clay nanotubes, photocatalysis, bioremediation of resistant pesticides, disinfectants, agricultural wastewater treatment, nanobarcode technology, quantum dots for staining bacteria and nanobiosensors. (3) Nanotechnological applications for agriculture, which includes nanolignodynamic metallic particles, photocatalysis, desalination, removal of heavy metals and wireless nanosensors. © 2017, Springer International Publishing AG.

Environmental Chemistry Letters

Applications of nanotechnology in agriculture and water quality management

Nano-titania is widely used in the food industry due to its efficient antimicrobial activity. However, the mechanism of microbial toxicity of nano-titania is poorly known. Here, nano-TiO2 has been fabricated by microwave-irradiation chemistry, a new method, and then tested for antimicrobial activity. Mutagenicity of nano-TiO2 was evaluated using Salmonella typhimurium histidine-auxotrophic strains. The reactive oxygen generation test was performed using 2,7-dichlorofluorescein diacetate dye. To test membrane permeabilization, E. coli cultures were grown in nutrient broth at an optical density of 0.3–0.5 at 610 nm, harvested by centrifugation at 11,000g for 10 min, washed and resuspended in 0.5 % NaCl solution. We also analyzed superoxide formation and membrane integrity, and we used scanning electron microscopy. Results show that nano-TiO2 has a minimum inhibitory concentration of 15 µg/mL, and a minimum bactericidal concentration of 20 µg/mL for E. coli. The bacterial inner wall was ruptured, and cytoplasmic content was released after 5 min of treatment in a dose-dependent manner. Notably, superoxide formation was not observed, which establishes the fact that reactive oxygen generation and alteration of membrane integrity, as well as permeability, is the major mechanism of antimicrobial activity of nano-TiO2. © 2016, Springer International Publishing Switzerland.

Titanium dioxide nanoparticles induce bacterial membrane rupture by reactive oxygen species generation

Nanoscience in Food and Agriculture 1 Sustainable Agriculture Reviews

Nanoagriculture and Water Quality Management

Nanotechnology has seen exponential growth in last decade due to its unique physicochemical properties; however, the risk associated with this emerging technology has withdrawn ample attention in the past decade. Nanotoxicity is majorly contributed to the small size and large surface area of nanomaterials, which allow easy dispersion and invasion of anatomical barriers in human body. Unique physio-chemical properties of nanoparticles make the investigation of their toxic consequences intricate and challenging. This makes it important to have an in-depth knowledge of different mechanisms involved in nanomaterials's action and toxicity. Nano-toxicity has various effects on human health and diseases as they can easily enter into the humans via different routes, mainly respiratory, dermal, and gastrointestinal routes. This also limits the use of nanomaterials as therapeutic and diagnostic tools. This review focuses on the nanomaterial-cell interactions leading to toxicological responses. Different mechanisms involved in nanoparticle-mediated toxicity with the main focus on oxidative stress, genotoxic, and carcinogenic potential has also been discussed. Different methods and techniques used for the characterization of nanomaterials in food and other biological matrices have also been discussed in detail. Nano-toxicity on different organs-with the major focus on the cardiac and respiratory system-have been discussed. Conclusively, the risk management of nanotoxicity is also summarized. This review provides a better understanding of the current scenario of the nanotoxicology, disease progression due to nanomaterials, and their use in the food industry and medical therapeutics. Briefly, the required rules, regulations, and the need of policy makers has been discussed critically.

Critical Reviews in Food Science and Nutrition

Nanomaterials in food and agriculture: An overview on their safety concerns and regulatory issues

Titanium dioxide nanoparticles are widely used in consumer products, paints and pharmaceutical preparations. They have been shown to induce cytotoxicity, genotoxicity and carcinogenicity—in vitro and in vivo. So far there is lack of standardized protocol for in silico analysis of nano-toxicological evaluations. In the present study, it was attempted to analyze the titanium dioxide nanoparticles-protein interaction through docking using AutoDock 4.0.5 software. Titanium dioxide nanoparticles with particle size of 1.09 nm were docked with different cellular proteins. Binding site area and volume has been determined by using CastP online server and docking has been performed at the active site as a pocket. The docked structures were analyzed for the most efficient binding with amino acids. It is the first study to report the interaction of titanium dioxide nanoparticles without any surface modification with proteins using docking analysis. The negative binding and docking energy inferred that the interaction of titanium dioxide nanoparticles with certain proteins is significant. Titanium dioxide nanoparticle shows significant interaction with intercellular adhesion molecule-1, P38 mitogen-activated protein kinases (P-38), placental growth factor and nuclear factor kappa-light-chain-enhancer of activated B cell proteins. Further, it has been observed that titanium dioxide nanoparticles show frequent interaction with proline, lysine as well as leusine. © 2015, The National Academy of Sciences, India.

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences

A Novel Approach to Evaluate Titanium Dioxide Nanoparticle–Protein Interaction Through Docking: An Insight into Mechanism of Action

As the nanoparticles (NPs) enter into the biological interface, they have to encounter immediate and first exposure to many proteins of different concentrations. The physicochemical interaction of NPs and proteins is greatly influenced not only by the number and type of proteins; but also the surface chemistry of NPs. To analyze the effects of NPs on proteins, the interaction between bovine serum albumin (BSA) and silver nanoparticles (AgNPs) at different concentrations were investigated. The interaction, BSA conformations, kinetics and adsorption were analyzed by UV-Visible spectrophotometer, dynamic light scattering (DLS), FT-IR spectroscopy and fluorescence quenching. DLS, FTIR and UV-visible spectrophotometric analysis confirms the interaction with minor alterations in size of the protein. Fluorescence quenching analysis confirms the side-on or end-on interaction of 1.5 molecules of BSA to AgNP. Further, pseudo-second order kinetics was determined with equilibrium contact-time of 30 min. The data of the present study determines the detailed evaluation of BSA adsorption on AgNP along with mechanism, kinetics and isotherm of the adsorption. © 2016 Elsevier Ireland Ltd.

Chemico-Biological Interactions

Bovine serum albumin interacts with silver nanoparticles with a “side-on” or “end on” conformation

The present study was carried out to fabricate the food grade Vitamin E acetate nanoemulsion using edible mustard oil and to evaluate its improved bioactivities. A food-grade Vitamin E acetate nanoemulsion was fabricated using the edible mustard oil and surfactant Tween-80. Flocculation was not observed for 15 days. The nanoemulsion was characterized for droplet morphology and size distribution using atomic force microscope and zetasizer, respectively. We observe a stable nanoemulsion of spherical morphology and a size distribution of 86.45 ± 3.61 nm. Further, the high-performance liquid chromatography method was used to determine the Vitamin E acetate concentration and encapsulation efficiency for the stable nanoemulsion. These nanoemulsions showed improved bioactivity, antioxidant, and antimicrobial activity and could be potentially used to increase the shelf life of fruit juice. Copyright © 2015 Taylor & Francis Group, LLC.

International Journal of Food Properties

Fabrication of Food Grade Vitamin E Nanoemulsion by Low Energy Approach, Characterization and Its Application

Titanium dioxide nanoparticles (TNPs) are widely used in the pharmaceutical and cosmetics industries. It is used for protection against UV exposure due to its light-scattering properties and high refractive index. Though TNPs are increasingly used, the synthesis of TNPs is tedious and time consuming; therefore, in the present study, microwave-assisted hybrid chemical approach was used for TNP synthesis. In the present study, we demonstrated that TNPs can be synthesized only in 2.5 h; however, the commonly used chemical approach using muffle furnace takes 5 h. The activity of TNP depends on the synthetic protocol; therefore, the present study also determined the effect of microwave-assisted hybrid chemical approach synthetic protocol on microbial and cytotoxicity. The results showed that TNP has the best antibacterial activity in decreasing order from Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The IC50 values of TNP for HCT116 and A549 were found to be 6.43 and 6.04 ppm, respectively. Cell death was also confirmed from trypan blue exclusion assay and membrane integrity loss was observed. Therefore, the study determines that the microwave-assisted hybrid chemical approach is time-saving; hence, this technique can be upgraded from lab scale to industrial scale via pilot plant scale. Moreover, it is necessary to find the mechanism of action at the molecular level to establish the reason for greater bacterial and cytotoxicological toxicity. [Figure not available: see fulltext.] © 2016, Springer-Verlag Berlin Heidelberg.

Environmental Science and Pollution Research

Microwave-irradiation-assisted hybrid chemical approach for titanium dioxide nanoparticle synthesis: microbial and cytotoxicological evaluation

A spectroscopic study on interaction between bovine serum albumin and titanium dioxide nanoparticle synthesized from microwave-assisted hybrid chemical approach

Journal	Data powered by TypesetJournal of Photochemistry and Photobiology B: Biology
Publisher	Data powered by TypesetElsevier BV
ISSN	1011-1344
Open Access	0