Introduction and Aim: : The primary mode of commutation of students from rural areas to reach students located in the urban areas is by bicycle. This necessitates the need for an ergonomically designed bicycle to avoid musculoskeletal disorders (MSDs), enhance comfort and improve the performance of the riders. The objective of the present study is to identify the discomfort level in different body segments by comparing riding posture on the rigid frame (RF) and sports (SP) cycle using Rehabilitation Bioengineering Group Pain Scale (RBGPS). Materials and Methods: Eighty school girls (Age group of 17 years) volunteers participated in this study. RBGPS pain scale is used to quantify recognized discomfort levels at ten body areas (i.e., neck, back, wrists/ hands, legs, and shoulders). Results: The participants who rode RF bicycle have found significantly (p < 0.05) high pain at the leg and mid-back portion of the body. Most of the schoolgirl is significantly (p < 0.05) affected by low back pain (LBP) due to SPbicycle riding. Conclusion: SP bicycle involved poor riding posture, where riders tend to bend forward which results in severe back pain. This study helps in improving the ergonomic standard of bicycle design for reducing MSDs for school girls. © 2018 Indian Association of Biomedical Scientists. All rights reserved.