Transition metal complexes have been exploited extensively to design new drugs due to their favorable elec-tronic environment and tunable geometry. These specific properties have proved to be advantageous for their versatile biological applications. Herein, we are focusing on synthesis of two ester based oxovanadium complexes, say. bis(butanoicacid-3-oxo-1,1-dimethylethylester)oxovanadium(IV) (1) and bis(dipropan-2-yl-propanedioate)bxovanadium(IV) (2). Complexes were characterized by UV spectroscopy, FTIR studies and Mass spectroscopic analysis. The complexes are assessed for chemical nuclease activity against plasmid pBR322 using gel electrophoresis. Further support towards binding mode is carried out by UV titration using calf thymus DNA. Docking studies are done to compare experimental and theoretical results. © 2015 Scientific Publishers. All rights reserved.