Bio-signal acquisition is a process that involves congregation of bio-signals that are very helpful in detecting a variety of ailments like epilepsy, sleep disorders, cardiac arrhythmia, best's disease, muscular and nervous-system disorders, dominant cystoids macular dystrophy, brain death and coma. Bio-signals can also be used for tracking the human kinesiology and also in enhancing human machine interaction. Bio-signal acquisition has a long history of use in healthcare and research, but there are certain practical factors that can affect the bio-signal acquisition. This work discusses about the practical factors affecting bio-signal acquisition and also proposes some of the possible engineering solutions to overcome these factors. © 2015 IEEE.