Whilst motorcycling is an activity of pleasure in most parts of the world, in India it is a regular mode of commuting. Incidence of fatigue is substantially higher among motorcycle riders than drivers of other modes of transport. The objective of this study was to detect physical fatigue due to motorcycle riding for an hour using surface electromyography (sEMG) and seat interface pressure. Twenty healthy male participants performed 60 min of motorcycle riding in a low traffic density environment. Muscle activity was recorded bilaterally from extensor carpi radialis (ECR), biceps brachii (BB), trapezius medial (TM), sternocleldomastoid (S) latissimus dorsi (LD) and erector spinae (ES) muscle groups. Interface seat pressure distribution was monitored using a pressure mapping system. Results showed that participants have significant (p < 0.05) physical fatigue in TM, LD and ES muscle groups during 60 min of motorcycle riding. Seat pressure distribution was found to be non-uniform during the course of motorcycling. Results suggest that the impact on local physical fatigue and seat discomfort are probably due to static seating demand and prolonged sitting posture balance required to ride the motorcycle for an hour. © 2013 Elsevier Ltd. All rights reserved.