Antioxidants protect cell from damage caused by unstable molecules known as free radicals. The deleterious reactions caused by free radicals are controlled by antioxidant through elimination of oxygen and thus scavenge free radicals. Ascorbic acid, glutathione, uric acid, carotenes, α-tocopherol and ubiquinol are some of the antioxidants synthesized in the body naturally. Naturally occurring antioxidants in leafy vegetables and seeds such as vitamin C, vitamin E, β-carotene, xanthophylls are preferred over synthetic antioxidants such as Butylated hydroxyl toluene (BHT) and Butylated hydroxyl anisole (BHA) as they cannot be used in food due to their carcinogenic nature. Carotenoid lutein reduces the oxidative damage or minimizes the damage due to oxidative stress by limiting the degree to which oxygen penetrates the membrane. Dietary lutein reduces inflammation and immunosuppression, and protects human cells against oxidant induced damage. In spite of the above said metabolic role of carotenoids, they cannot be produced inside the animal bodies. So they have to be obtained through the diet. Hence, there is a need for isolation of antioxidants particularly carotenoids from natural products. The aim of our study is to extract lutein from various sources, estimate its yield in different solvents and study the effect of standing time on concentration of lutein in different solvents. This study focuses on extraction and estimation of lutein from the selected plants to determine the most suitable source which offers high yield and its affordability.