Electrospinning is an emerging tool and promising method to fabricate polymer nanofibers. The aim of this work was to fabricate electrospun polyurethane mats reinforced with murivenna oil for wound dressings. The scanning electron microscopy (SEM) micrographs showed the fiber diameter of nanocomposites in the range of 740 ± 160 nm and found to be decreased compared to pure polyurethane. Surface of nanocomposites was analyzed by Fourier transform infrared spectroscopy (FTIR) insinuated the interactions between PU and murivenna oil by the formation of hydrogen bond and changes in the characteristics peaks. Contact angle of the PU incorporated murivenna oil showed a decrease in its value compared to pure PU indicating the increased wettability and hydrophilic nature. The thermal degradation and stability of fabricated composites was found be enhanced compared to pure PU. The surface morphology through atomic force microscopy (AFM) analysis showed a change in surface roughness due to presence of murivenna oil in the polymer matrix. In blood compatibility results, both activated partial thromboplastin time (APTT) and prothrombin time (PT) were delayed due to improved surface properties and the addition of murivenna oil in the PU matrix. Compared to pure PU, the hemolysis assay of the PU incorporated murivenna oil showed a significant decrease in the percentage of lysis of red blood cells indicating better blood compatibility. Following the results, it was confirmed that fabricated novel scaffolds having better physicochemical and enhanced blood compatibility properties may be utilized for wound dressing. © 2017 Elsevier Ltd